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KMID : 0364820130490030253
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Korean Journal of Microbiology
2013 Volume.49 No. 3 p.253 ~ p.261
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Incubation Conditions and Physico-Chemical Factors Affecting Aflatoxin B1 Binding of Lactic Acid Bacteria
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Lim Sung-Mee
Ahn Dong-Hyun
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Abstract
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The purpose of this study was to investigate the aflatoxin B1 binding of lactic acid bacteria (LAB) isolated from
Korean traditional soybean paste and to evaluate the effect of incubation conditions and physico-chemical factors on
the binding ability of LAB to this mutagen. The amount of aflatoxin B1 bound by Enterococcus faecium DJ22,
Lactobacillus fermentum DJ35, Lactobacillus rhamnosus DJ42, and Lactobacillus pentosus DJ47 was strain specific
with the percent bound ranging from 19.3% to 52.1%. However, Enterococcus faecalis DJ14, Lactobacillus panis
DJ29, and Pediococcus halophilus DJ50 strains did not exhibit any of the binding ability to aflatoxin B1. For most
strains, the binding ability was significantly affected by the environmental conditions such as the aflatoxin B1 level,
incubation time and temperature, and the initial cell count of LAB. The stability of the aflatoxin B1-bacteria
complexes was significantly more unstable after washing. In addition, the binding stability between viable and
nonviable cells was not statistically significant. Treatment with heating, acidic pH, α-amylase, protease, lysozyme, or
sodium metaperiodate caused a significant (P<0.05) decrease in aflatoxin B1 binding for the tested strains,
suggesting that carbohydrates or proteins in the cell walls may be involved in aflatoxin B1 binding ability. Since the
aflatoxin B1 binding of LAB was significantly reduced (P<0.05) by the pretreatment of the urea, the binding force
observed in this study may have resulted from hydrophobic interaction.
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KEYWORD
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aflatoxin B1 binding, lactic acid bacteria, soybean paste
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